| Primary Identifier | MGI:3760542 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Rapgef6 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:126164 does not mention whether the null allele of Pou5f1 is also present in the line of mice created. |
| molecularNote | A loxP site was inserted upstream of exon 21 and a floxed neo cassette downstream of exon 21 to create a floxed allele in EB3 ES cells. Insertion was confirmed by Southern blot analysis. EB3 cells carry one allele of Pou5f1 that has been inactivated by targeted integration of E14Tg2a ES cells with an IRESBSDpA selection cassette. |
