| First Author | Takahashi K | Year | 2021 |
| Journal | Int J Mol Sci | Volume | 22 |
| Issue | 21 | PubMed ID | 34768987 |
| Mgi Jnum | J:314734 | Mgi Id | MGI:6826531 |
| Doi | 10.3390/ijms222111557 | Citation | Takahashi K, et al. (2021) Retinal Degeneration and Microglial Dynamics in Mature Progranulin-Deficient Mice. Int J Mol Sci 22(21) |
| abstractText | Progranulin (PGRN) is a secreted glycoprotein that regulates numerous cellular processes. The role of PGRN as a regulator of lysosomes has recently received attention. The purpose of this study was to characterize the retinal phenotype in mature PGRN knockout (Grn(-/-)) mice. The a-wave amplitude of scotopic electroretinogram and outer nuclear thickness were significantly reduced at 6 months of age in Grn(-/-) mice compared to wild-type (Grn(+/+)) mice. In Grn(-/-) mice, retinal microglial cells accumulated on the retinal pigment epithelium (RPE) apical layer, and the number of infiltrated microglia and white fundus lesions between 2 and 6 months of age showed a close affinity. In Grn(+/+) mice, PGRN was located in the retina, while the strongest PGRN signals were detected in the RPE-choroid. The different effects of PGRN deficiency on the expression of lysosomal proteins between the retina and RPE-choroid were demonstrated. Our data suggest that the subretinal translocation of microglia is a characteristic phenotype in the retina of mature PGRN knockout mice. The different effects of PGRN deficiency on the expression of lysosomal proteins between the retina and RPE-choroid might modulate microglial dynamics in PGRN knockout mice. |
