| Primary Identifier | MGI:3762626 | Allele Type | Targeted |
| Attribute String | Dominant negative, Humanized sequence | Gene | Krt75 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 1 was replaced with a version of exon 1 in which the asparagine at position 159 was deleted. A floxed cre cassette that was inserted downstream of exon 1 for selection purposes was removed by cre-mediated recombination using a transient cre expressing plasmid. The authors state that this mutation should produce a dominant negative protein. Expression of only the modified transcript was confirmed by RT-PCR analysis. |
