| Primary Identifier | MGI:3762911 | Allele Type | Targeted |
| Attribute String | Not Specified | Gene | Irak4 |
| Transmission | Germline | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was constructed where lysines 213 and 214 that are part of the ATP-binding pocket of the protein were mutated to methionines (KK213-214MM). An FRT-flanked neomycin selection cassette was also included and then subsequently removed leaving behind a single FRT site. Western blots confirmed the mutant protein has similar expression to wild-type protein. Abolition of kinase activity of the protein was confirmed by an in vitro assay. |
