| First Author | Diener N | Year | 2021 |
| Journal | Proc Natl Acad Sci U S A | Volume | 118 |
| Issue | 38 | PubMed ID | 34526403 |
| Mgi Jnum | J:310657 | Mgi Id | MGI:6763919 |
| Doi | 10.1073/pnas.2111234118 | Citation | Diener N, et al. (2021) Posttranslational modifications by ADAM10 shape myeloid antigen-presenting cell homeostasis in the splenic marginal zone. Proc Natl Acad Sci U S A 118(38):e2111234118 |
| abstractText | The spleen contains phenotypically and functionally distinct conventional dendritic cell (cDC) subpopulations, termed cDC1 and cDC2, which each can be divided into several smaller and less well-characterized subsets. Despite advances in understanding the complexity of cDC ontogeny by transcriptional programming, the significance of posttranslational modifications in controlling tissue-specific cDC subset immunobiology remains elusive. Here, we identified the cell-surface-expressed A-disintegrin-and-metalloproteinase 10 (ADAM10) as an essential regulator of cDC1 and cDC2 homeostasis in the splenic marginal zone (MZ). Mice with a CD11c-specific deletion of ADAM10 (ADAM10(DeltaCD11c)) exhibited a complete loss of splenic ESAM(hi) cDC2A because ADAM10 regulated the commitment, differentiation, and survival of these cells. The major pathways controlled by ADAM10 in ESAM(hi) cDC2A are Notch, signaling pathways involved in cell proliferation and survival (e.g., mTOR, PI3K/AKT, and EIF2 signaling), and EBI2-mediated localization within the MZ. In addition, we discovered that ADAM10 is a molecular switch regulating cDC2 subset heterogeneity in the spleen, as the disappearance of ESAM(hi) cDC2A in ADAM10(DeltaCD11c) mice was compensated for by the emergence of a Clec12a(+) cDC2B subset closely resembling cDC2 generally found in peripheral lymph nodes. Moreover, in ADAM10(DeltaCD11c) mice, terminal differentiation of cDC1 was abrogated, resulting in severely reduced splenic Langerin(+) cDC1 numbers. Next to the disturbed splenic cDC compartment, ADAM10 deficiency on CD11c(+) cells led to an increase in marginal metallophilic macrophage (MMM) numbers. In conclusion, our data identify ADAM10 as a molecular hub on both cDC and MMM regulating their transcriptional programming, turnover, homeostasis, and ability to shape the anatomical niche of the MZ. |
