| Primary Identifier | MGI:3763534 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Egfr |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd-Hprt1<b-m3> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector in which the promoter and the first exon of the gene are flanked by loxP sites was constructed. The upstream loxP site with a diagnostic XmaCI restriction site was placed into a BSMI site, and the downstream loxP site was cloned into the SexA1 site. A frt-flanked neomycin cassette was placed between exon 1 and the downstream loxP site. Recombination was confirmed through PCR and Southern blot analysis. |
