Primary Identifier | MGI:3762772 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Pdcd1lg2 |
Transmission | Germline | Strain of Origin | 129S/SvEvBrd |
Is Recombinase | false | Is Wild Type | false |
Project Collection | Lexicon |
molecularNote | By homologous recombination a loxP site was inserted 5' of exon2, followed by a NeoR cassette and a 3' loxP site. The resulting mutant mice, Pdcd1lg2tm1Lex were crossed with protamine-Cre transgenic mice to delete exon 2 containing the translational start site. Flow cytometric protein expression analysis confirmed absence of targeted protein expression. |