| Primary Identifier | MGI:3764892 | Allele Type | Targeted |
| Gene | Ptger1 | Transmission | Germline |
| Strain of Origin | 129S6/SvEvTac | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Three point mutations were introduced into exon 2 using a "hit and run" type vector. One mutation (C795T; numbering based on MGI ID: D16388) introduces an in-frame stop codon (R242X) that is predicted to stop translation at the beginning of the third cytoplasmic loop of the receptor, resulting in a nonfunctional protein. The other two mutations (C798G; G799A) introduce an EcoRI site that is used for genotyping. Sequencing of mRNA confirmed expression of the mutant allele in homozygous mice. Expression of the PKN1 gene present on the opposite antisense strand was unaffected as determined by immunoprecipitation of brain and kidney lysates. |
