| Primary Identifier | MGI:3764940 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Apc |
| Transmission | Germline | Strain of Origin | 129S4/SvJae |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | One loxP site was inserted into intron 13 and the other, with a PGK-neo cassette, into intron 14. Exon 14 was excised by Cre mediated recombination, resulting in a nonsense frame-shift mutation. Excision of the exon was confirmed by RT-PCR and sequencing analysis of mRNA isolated from the large intestine. Western blot detected the truncated product (70 kDa). |
