| First Author | Komori T | Year | 2018 |
| Journal | Sci Rep | Volume | 8 |
| Issue | 1 | Pages | 2612 |
| PubMed ID | 29422532 | Mgi Jnum | J:263002 |
| Mgi Id | MGI:6163307 | Doi | 10.1038/s41598-018-21000-0 |
| Citation | Komori T, et al. (2018) Type IV collagen alpha6 chain is a regulator of keratin 10 in keratinization of oral mucosal epithelium. Sci Rep 8(1):2612 |
| abstractText | Keratinized mucosa is of fundamental importance to maintain healthy gingival tissue, and understanding the mechanisms of oral mucosa keratinization is crucial to successfully manage healthy gingiva. Previous studies have shown a strong involvement of the basement membrane in the proliferation and differentiation of epithelial cells. Therefore, first, to identify the keratinized mucosa-specific basement membrane components, immunohistochemical analysis for the six alpha chains of type IV collagen was performed in 8-week-old mice. No difference in the expression pattern of type IV collagen alpha1(IV) and alpha2(IV) chains was observed in the keratinized and non-keratinized mucosa. Interestingly, however, type IV collagen alpha5(IV) and alpha6(IV) chains specifically were strongly detected in the keratinized mucosa. To analyze the functional roles of the type IV collagen isoform alpha6(IV) in oral mucosa keratinization, we analyzed Col4a6-knockout mice. Epithelial developmental delay and low levels of KRT10 were observed in new-born Col4a6-knockout mice. Additionally, in vitro experiments with loss-of function analysis using human gingival epithelial cells confirmed the important role of alpha6(IV) chain in epithelial keratinization. These findings indicate that alpha112:alpha556 (IV) network, which is the only network that includes the alpha6(IV) chain, is one regulator of KRT10 expression in keratinization of oral mucosal epithelium. |
