| First Author | Daigle TL | Year | 2018 |
| Journal | Cell | Volume | 174 |
| Issue | 2 | Pages | 465-480.e22 |
| PubMed ID | 30007418 | Mgi Jnum | J:260362 |
| Mgi Id | MGI:6151054 | Doi | 10.1016/j.cell.2018.06.035 |
| Citation | Daigle TL, et al. (2018) A suite of transgenic driver and reporter mouse lines with enhanced brain cell type targeting and functionality. Cell 174(2):465-480.e22 |
| abstractText | Modern genetic approaches are powerful in providing access to diverse types of neurons within the mammalian brain and greatly facilitating the study of their function. We here report a large set of driver and reporter transgenic mouse lines, including 23 new driver lines targeting a variety of cortical and subcortical cell populations and 26 new reporter lines expressing an array of molecular tools. In particular, we describe the TIGRE2.0 transgenic platform and introduce Cre-dependent reporter lines that enable optical physiology, optogenetics, and sparse labeling of genetically-defined cell populations. TIGRE2.0 reporters broke the barrier in transgene expression level of single-copy targeted-insertion transgenesis in a wide range of neuronal types,along with additional advantage of a simplified breeding strategy compared to our first-generation TIGRE lines. These novel transgenic lines greatly expand the repertoire of high-precision genetic tools available to effectively identify, monitor, and manipulate distinct cell types in the mouse brain. |
