Primary Identifier | MGI:3766933 | Allele Type | Targeted |
Attribute String | Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | 129S4/SvJaeSor |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting construct with the SA-attB-PGKneo 4xpA-attP-lacZ-pA cassette, in which the STOP fragment is flanked by attB and attP sites (phi31 recognition sites), was inserted into the Gt(ROSA)26Sor. Expression of the lacZ gene is blocked by a STOP fragment (flanked by attB and attP sites) placed between the lacZ sequence and the Gt(ROSA)26Sor promoter. PhiC31o-mediated recombination will excise the STOP and allow lacZ transcription. |