| First Author | Sato T | Year | 2009 |
| Journal | Nature | Volume | 459 |
| Issue | 7244 | Pages | 262-5 |
| PubMed ID | 19329995 | Mgi Jnum | J:224358 |
| Mgi Id | MGI:5662058 | Doi | 10.1038/nature07935 |
| Citation | Sato T, et al. (2009) Single Lgr5 stem cells build crypt-villus structures in vitro without a mesenchymal niche. Nature 459(7244):262-5 |
| abstractText | The intestinal epithelium is the most rapidly self-renewing tissue in adult mammals. We have recently demonstrated the presence of about six cycling Lgr5(+) stem cells at the bottoms of small-intestinal crypts. Here we describe the establishment of long-term culture conditions under which single crypts undergo multiple crypt fission events, while simultanously generating villus-like epithelial domains in which all differentiated cell types are present. Single sorted Lgr5(+) stem cells can also initiate these cryptvillus organoids. Tracing experiments indicate that the Lgr5(+) stem-cell hierarchy is maintained in organoids. We conclude that intestinal cryptvillus units are self-organizing structures, which can be built from a single stem cell in the absence of a non-epithelial cellular niche. |
