Primary Identifier | MGI:3764690 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Prkn |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 3 was replaced with a neo-loxP cassette resulting in a frameshift mutation after amino acid 57. The shortened transcript was confirmed by RT-PCR analysis on brain tissue. The lack of protein production was confirmed by western blot analysis on total brain protein using a polyclonal antibody against the N-terminus. |