| First Author | Hefendehl JK | Year | 2011 |
| Journal | J Neurosci | Volume | 31 |
| Issue | 2 | Pages | 624-9 |
| PubMed ID | 21228171 | Mgi Jnum | J:180835 |
| Mgi Id | MGI:5307960 | Doi | 10.1523/JNEUROSCI.5147-10.2011 |
| Citation | Hefendehl JK, et al. (2011) Long-term in vivo imaging of beta-amyloid plaque appearance and growth in a mouse model of cerebral beta-amyloidosis. J Neurosci 31(2):624-9 |
| abstractText | Extracellular deposition of the amyloid-beta peptide (Abeta) in the brain parenchyma is a hallmark lesion of Alzheimer's disease (AD) and a predictive marker for the progression of preclinical to symptomatic AD. Here, we used multiphoton in vivo imaging to study Abeta plaque formation in the brains of 3- to 4-month-old APPPS1 transgenic mice over a period of 6 months. A novel head fixation system provided robust and efficient long-term tracking of single plaques over time. Results revealed an estimated rate of 35 newly formed plaques per cubic millimeter of neocortical volume per week at 4-5 months of age. At later time points (i.e., in the presence of increasing cerebral beta-amyloidosis), the number of newly formed plaques decreased. On average, both newly formed and existing plaques grew at a similar growth rate of 0.3 mum (radius) per week. A solid knowledge of the dynamics of cerebral beta-amyloidosis in mouse models provides a powerful tool to monitor preclinical Abeta targeting therapeutic strategies and eases the interpretation of diagnostic amyloid imaging in humans. |
