| Primary Identifier | MGI:3768257 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Gpr83 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A loxP element was inserted 347 bp upstream of exon 3, and a cassette containing a loxP site and a FRT-flanked neomycin cassette was inserted 703 bp downstream of exon 4. Chimeric mice were first mated to FLP deleter mice to remove the neomycin cassette, and subsequently to Cre-transgenic mice to remove the floxed alleles. Gene deletion was confirmed by lack of expression in regulatory T cells as determined by quantitative RT-PCR. |
