Primary Identifier | MGI:3767676 | Allele Type | Transgenic |
Attribute String | Inducible, Inserted expressed sequence | Gene | Tg(tetO-Creb1*S133A)1Rdu |
Strain of Origin | C57BL/6 x SJL | Induced With | doxycycline/tetracycline |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A rat Creb1 cDNA was mutated at codon 133 to alter the endcoded serine to an alanine. In addition, a FLAG-tag peptide was added at the N-terminus to distinguish the expressed protein from the endogenous protein. The modified cDNA was cloned into an expression vector containing a tetracycline responsive element (TetOP) and a CMV minimal promoter. |