| Primary Identifier | MGI:3771368 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Pou5f1 |
| Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | When treated with specific transcription factors (Oct4, Sox2, c-Myc and Klf4), some Oct4-neo murine embryonic fibroblasts (MEFs) have the properties of induced pluripotent stem (iPS) cells. Such iPS cells have the DNA methylation, gene expression and chromatin state of embryonic stem cells and can form viable chimeras, contribute to the germ line, and generate live late-term embryos when injected into tetraploid blastocysts. |
| molecularNote | The Oct4-IRES-GFPneo targeting vector was designed to insert an IRES-GFPneo fusion cassette downstream of exon 5. While sufficient neomycin-resistance activity is observed in embryonic stem cells, no visible GFP fluorescence is reported. |
