| Primary Identifier | MGI:3772051 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Trim24 |
| Transmission | Germline | Strain of Origin | 129/Sv |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Exon 4 was floxed by introducing a loxP site upstream of the exon and inserting a loxP flanked PGK/TK neomycin cassette downstream of the exon. Chimeric mice were generated, crossed with a Cre-deleter strain and offspring were selected that had both exon 4 and the neo selection cassette removed. The deletion was also predicted to generate a frameshift mutation with a premature stop codon. Gene inactivation was confirmed by Western blot analysis of liver and testis protein. |
