Primary Identifier | MGI:3772862 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Tsix |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
description | Chimeric mice were bred with Tsixtm1Enl mice to produce null male mice. |
molecularNote | A multiple polyadenylation sequence flanked by splice donor and acceptor sites was inserted into exon 4 of Tsix in its orientation. The insertion site is also within exon 1 of Xist on the other strand. The absence of transcript expression from Xist and Tsix was confirmed by RT-PCR. |