Primary Identifier | MGI:3772898 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Robo1 |
Transmission | Germline | Strain of Origin | 129S/SvEv-Gpi1<c> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | In a two step integration process a hyg cassette with a 5' loxP site was inserted upstream of exon 1 and a puromycin resistance cassette with a 3' loxP site was inserted upstream of exon 22. Cre-mediated recombination was used to delete exons 1 through 21. The absence of protein expression was confirmed by immunohistochemistry. |