| Primary Identifier | MGI:3773307 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Igh |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A variable heavy chain gene segment belonging to the VH558 family along with 1.3 kb of upstream sequence and 500 bp of downstream sequence was knocked into a region of the IgH complex that lies 500 bp upstream of the most proximal D segment, DFL16.1. A loxP-flanked neomycin targeting cassette included in the targeting was subsequently removed by crossing chimeric mice with Cre expressing transgenic mice. Targeting events were confirmed through Southern blotting and restriction mapping. |
