| Primary Identifier | MGI:3774153 | Allele Type | Targeted |
| Gene | Mitf | Transmission | Germline |
| Strain of Origin | 129S1/Sv-Oca2<+> Tyr<+> Kitl<+> | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | This allele resulted from an unplanned intragenic duplication event that occurred during a targeted mutation of the gene. The targeted mutation was within exon 2B to change the coding sequence for amino acid 73 from serine to alanine (S73A) and a silent nucleotide change 10bp upstream to generate a diagnostic restriction site. A floxed neomycin selection cassette was also inserted into intron 2. During recombination, 85,222 bp of wild-type gene sequence duplicated and inserted after exon 4 so that 7 exons (1H through 4) are repeated. Restriction digest and Southern blotting confirmed the presence of the targeted mutation and the duplication event. |
