| Primary Identifier | MGI:3775825 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Fstl4 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A GFP-neo cassette with sequence encoding a 14 amino acid linker was inserted in frame into exon 2 replacing most of it. Only the first 4 amino acids of exon 2, the first coding exon, are expressed. The presence of the fusion transcript and protein was confirmed by RT-PCR and immunohistochemistry, respectively. The absence of the endogenous transcript was confirmed by RT-PCR. This is a null allele. |
