| Primary Identifier | MGI:3776665 | Allele Type | Spontaneous |
| Attribute String | Null/knockout | Gene | Del(MTmt-Tk-mt-Nd5)1Jiha |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | Mice containing this deletion are referred to as mito-mice. Two methods were used to generate mice including transplantation of cytoplasm from enucleated cybrid cells into donor pronuclear stage embryos (J:65025) and fusion of enucleated cybrid cells to mitochondrially depleted TT2F ES cells (J:132407). |
| molecularNote | C2 cells depleted of their mitochondrial DNA were fused with synaptosomal fractions from brain tissue of inbred C57BL/6J mice. One clone possessed a 4696bp deletion that spanned from mt-Tk to mt-Nd5 and encompassed 6 tRNA genes and 7 structural genes. This deletion arose spontaneously and does not have direct repeats surrounding the breakpoint. This allele is maintained within cells that contain 30% mutant mitochondria. |
