| Primary Identifier | MGI:3777800 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(CD2-Tbx21)727Staka |
| Strain of Origin | (C57BL/6 x DBA/2)F1 | Is Recombinase | false |
| Is Wild Type | false |
| description | This particular transgenic line is not specifically described in the literature. Ishizaki et al. (J:133041) report generation of Tg lines 725 and 731 and crossing them to C57BL/6 for 4 generations. Kiwamoto et al. (J:128630) do not identify the transgenic lines used and says they were backcrossed to BALB/c for 8 generations. |
| molecularNote | A full-length, 2.5-kb mouse Tbx21 cDNA was inserted into the VA CD2 expression vector between, 5', the promoter, ATG-free mutant first exon and first intron of the human CD2 gene and 3', also from the CD2 gene, ~500 bp of 3' untranslated and flanking DNA including two polyadenylation signals and the 3' locus control region. A lacZ reporter gene was shown to be expressed from this vector in mature peripheral CD4+CD8- and CD4-CD8+ SP T lymphocytes and in peripheral CD4-CD8- DN T cells. |
