| Primary Identifier | MGI:3777625 | Allele Type | Targeted |
| Attribute String | Conditional ready, Reporter | Gene | Gt(ROSA)26Sor |
| Transmission | Germline | Strain of Origin | 129 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A dual recombinase-responsive indicator cassette was knocked into the gene locus. This cassette consisted of (upstream to downstream): a cytomegalovirus enhancer/chicken beta-actin promoter; a splice acceptor; a loxP-flanked PGK-neomycin resistance cassette followed by four consecutive polyadenylation sequences; a FRT-flanked nuclear lacZ encoding gene followed by five consecutive polyadenylation sequences; and a bicistronic element encoding for wheat germ agglutinin (WGA) and a farnesylated, membrane-localized form of eGFP. This allele expresses nlacZ after cre-mediated excision of the neo cassette and turns off nlacZ and turns on WGA and eGFP expression after both cre- and Flp-recombinases are expressed. |
