| Primary Identifier | MGI:3777637 | Allele Type | Targeted |
| Attribute String | Inducible, Recombinase | Gene | Chat |
| Transmission | Germline | Strain of Origin | 129 |
| Induced With | tamoxifen | Is Recombinase | true |
| Is Wild Type | false |
| description | ES cell line = JHMI-ES1. |
| molecularNote | An IRES-creER cassette, a tetracycline resistance gene, and a Frt-flanked neomycin selection marker were all knocked in to the 3' UTR of the gene. The neomycin selection marker was removed by crossing to a C57BL/6 background Flp recombinase strain. Flp was then bred out of the line. When crossed with a Tg(CAG-Bgeo/ALPP)1Lbe reporter strain and given tamoxifen, cre expression was detected in amacrine cells of the retina, and in a sparse number of cholinergic neurons in the brain. |
