Primary Identifier | MGI:3777591 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Rap1a |
Transmission | Germline | Strain of Origin | 129S/SvEv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 2 and a loxP-flanked neomycin selection cassette was inserted downstream of exon 3. Transient expression of cre recombinase removed the neomycin cassette, leaving exons 2 and 3 flanked by loxP sites. Correct targeting was confirmed through Southern blots. |