Primary Identifier | MGI:3777593 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Rap1b |
Transmission | Germline | Strain of Origin | 129S/SvEv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP-flanked neomycin selection cassette was inserted upstream of exon 1 with an additional loxP site inserted downstream of the exon. Transient expression of cre recombinase removed the neomycin selection cassette leaving exon 1 flanked by loxP sites. Correct targeted was confirmed through Southern blotting. |