| First Author | Riley DRJ | Year | 2020 |
| Journal | Int J Mol Sci | Volume | 21 |
| Issue | 1 | PubMed ID | 31948107 |
| Mgi Jnum | J:297130 | Mgi Id | MGI:6471973 |
| Doi | 10.3390/ijms21010356 | Citation | Riley DRJ, et al. (2020) Coronin 1 Is Required for Integrin beta2 Translocation in Platelets. Int J Mol Sci 21(1):356 |
| abstractText | Remodeling of the actin cytoskeleton is one of the critical events that allows platelets to undergo morphological and functional changes in response to receptor-mediated signaling cascades. Coronins are a family of evolutionarily conserved proteins implicated in the regulation of the actin cytoskeleton, represented by the abundant coronins 1, 2, and 3 and the less abundant coronin 7 in platelets, but their functions in these cells are poorly understood. A recent report revealed impaired agonist-induced actin polymerization and cofilin phosphoregulation and altered thrombus formation in vivo as salient phenotypes in the absence of an overt hemostasis defect in vivo in a knockout mouse model of coronin 1. Here we show that the absence of coronin 1 is associated with impaired translocation of integrin beta2 to the platelet surface upon stimulation with thrombin while morphological and functional alterations, including defects in Arp2/3 complex localization and cAMP-dependent signaling, are absent. Our results suggest a large extent of functional overlap among coronins 1, 2, and 3 in platelets, while aspects like integrin beta2 translocation are specifically or predominantly dependent on coronin 1. |
