| First Author | Osawa K | Year | 2023 |
| Journal | J Hard Tissue Biol | Mgi Jnum | J:343218 |
| Mgi Id | MGI:7564503 | Doi | 10.2485/jhtb.32.213 |
| Citation | Osawa K, et al. (2023) Morphological study for the Osteocytes in Podoplanin-Conditional knockout mice. J Hard Tissue Biol |
| abstractText | We generated podoplanin-conditional knockout mice where the floxed podoplanin exon3 was deleted by the Dmp-1-driven Cre (Dmp1-Cre;PdpnÎ/Î) and investigated the cell process elongation of podoplanin-deficient mouse osteocyte in vitro and in vivo. The expression of podoplanin is found in odontoblasts while not observed in odontoblasts of Dmp1-Cre; PdpnÎ/Î mice, indicating that the conditional knockout of podoplanin in Dmp1-expressing cells in Dmp1-Cre;PdpnÎ/Î mice is successful. There were no differences in the growth of wild-type and Dmp1-Cre;PdpnÎ/Î mice, and no differences in calci-fication and alkaline phosphatase activity in cultured calvarial osteoblasts of the wild-type and Dmp1-Cre;PdpnÎ/Î mice, in total this suggests that the podoplanin-cKO has no effect on generation of the bone. The cell process elongation was sup-pressed in cultured calvarial osteoblasts of Dmp1-Cre;PdpnÎ/Î mice compared with wild-type mice. In the electron micro-scopic study, there were no morphological differences in bone matrix formation and osteocyte distribution in Dmp1-Cre; PdpnÎ/Î and wild-type mice, whereas the cell process formation was sparser and the network with neighboring cells was more deficient in Dmp1-Cre;PdpnÎ/Î mice than in wild-type mice. In the quantitative analysis, the number and thickness of the cell processes were significantly smaller and thinner in Dmp1-Cre;PdpnÎ/Î mice than in wild-type mice. This could sug-gest that podoplanin plays a role in the formation of the osteocyte network created by the cell process elongation. |
