| First Author | Engler P | Year | 1991 |
| Journal | Cell | Volume | 65 |
| Issue | 6 | Pages | 939-47 |
| PubMed ID | 2044153 | Mgi Jnum | J:11225 |
| Mgi Id | MGI:59665 | Doi | 10.1016/0092-8674(91)90546-b |
| Citation | Engler P, et al. (1991) A strain-specific modifier on mouse chromosome 4 controls the methylation of independent transgene loci. Cell 65(6):939-47 |
| abstractText | A transgene, pHRD, is highly methylated in 12 independent mouse lines when in a C57BL/6 strain background, but becomes progressively less methylated when bred into a DBA/2 background. Transgenes inherited from the mother are generally more methylated; however, this parental effect disappears following continued breeding into the nonmethylating strain. Mapping experiments using BXD recombinant inbred mice as well as other inbred strains indicate that a single strain-specific modifier (Ssm-1) linked to, but distinct from, Fv-1 is responsible for the strain effect. In addition to the methylated and unmethylated transgenic phenotypes, certain mice exhibit a partial methylation pattern that is a consequence of an unusual cellular mosaicism. The pHRD transgene, containing target sequences for the V(D)J recombinase, undergoes site-specific recombination only in lymphoid tissues. This V-J joining is restricted primarily to unmethylated transgene copies. |
