Primary Identifier | MGI:3790807 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | E2f6 |
Transmission | Germline | Strain of Origin | 129S4/SvJae |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exons 4 through 8, encoding the DNA binding, leucine zipper, and marked box domains were replaced via homologous recombination with a floxed PGK-neo cassette that includes a stop codon at the beginning. Western blot analysis of homozygous MEFs confirmed the absence of protein expression. |