Primary Identifier | MGI:3790328 | Allele Type | Targeted |
Attribute String | Null/knockout, Reporter | Gene | Sema3f |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
description | ES cells = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. |
molecularNote | A lacZ cassette was inserted into exon 3. Cre-mediated recombination removed the floxed selection cassette inserted downstream of exon 3. The absence of transcript expression was confirmed by northern blot and in situ hybridization. This was generated from Sema3ftm2Atai. |