| First Author | Higginbotham H | Year | 2004 |
| Journal | Transgenic Res | Volume | 13 |
| Issue | 2 | Pages | 155-64 |
| PubMed ID | 15198203 | Mgi Jnum | J:91267 |
| Mgi Id | MGI:3046366 | Doi | 10.1023/b:trag.0000026071.41735.8e |
| Citation | Higginbotham H, et al. (2004) Transgenic mouse line with green-fluorescent protein-labeled Centrin 2 allows visualization of the centrosome in living cells. Transgenic Res 13(2):155-64 |
| abstractText | The centrosome plays diverse roles throughout the cellular mitotic cycle and in post-mitotic cells. Analysis of centrosome position and dynamics in living murine cells has been limited due to a lack of adequate reporters and currently requires either cell fixation/immunostaining or transfection with centrosome reporters. Here we describe the generation and characterization of a transgenic mouse line that constitutively expresses green fluorescent protein-labeled Centrin-2 (GFP-CETN2). The phenotype of the mouse is indistinguishable from wild-type and it displays a single pair of fluorescent centrioles in cells of every organ and time point examined. This model will be helpful for visualizing the centrosome in multiple experimental conditions. |
