| First Author | Barr JL | Year | 2020 |
| Journal | Neuroscience | Volume | 425 |
| Pages | 101-111 | PubMed ID | 31783102 |
| Mgi Jnum | J:285583 | Mgi Id | MGI:6391866 |
| Doi | 10.1016/j.neuroscience.2019.10.055 | Citation | Barr JL, et al. (2020) Glycogen Synthase Kinase 3beta in the Ventral Hippocampus is Important for Cocaine Reward and Object Location Memory. Neuroscience 425:101-111 |
| abstractText | The ventral hippocampus is a component of the neural circuitry involved with context-associated memory for reward and generation of appropriate behavioral responses to context. Glycogen synthase kinase 3 beta (GSK3beta) has been linked to the maintenance of synaptic plasticity, contextual memory retrieval, and is involved in the reconsolidation of cocaine-associated contextual memory. In this study, the effects of targeted downregulation of GSK3beta in the ventral hippocampus were examined on a series of behavioral tests for assessing drug reward-context association and non-reward related memory. The Cre/loxP site-specific recombination system was used to knockdown GSK3beta through bilateral stereotaxic delivery of an adeno-associated virus expressing Cre-recombinase (AAV-Cre) into the ventral hippocampus of adult mice homozygous for a floxed GSK3beta allele. GSK3beta floxed mice injected with AAV-Cre had a loss of 56-75% of GSK3beta in the ventral hippocampus and displayed diminished development of cocaine conditioned place preference, but not morphine place preference as compared with wild-type mice injected with AAV-Cre or GSK3beta floxed mice injected with a control virus, AAV-GFP. Impaired object location memory was observed in mice with GSK3beta downregulation in the ventral hippocampus, but novel object recognition remained intact. These results indicate that GSK3beta signaling in the ventral hippocampus is differentially involved in the formation of place-drug reward association dependent upon drug class. Additionally, ventral hippocampal GSK3beta signaling is important in detection of discrete spatial cues, but not recognition memory for objects. |
