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Allele : Tg(Cspg4-DsRed.T1)1Akik transgene insertion 1, Akiko Nishiyama

Primary Identifier  MGI:3796063 Allele Type  Transgenic
Attribute String  Reporter Gene  Tg(Cspg4-DsRed.T1)1Akik
Strain of Origin  (C57BL/6 x SJL)F1 Is Recombinase  false
Is Wild Type  false
molecularNote  The transgene construct consisted of the 208 kb C57BL/6J mouse bacterial artificial chromosome (BAC) RP23-309G21, containing the entire NG2 (Cspg4) gene (and 60 kbp of 5' and 114 kbp of 3' flanking sequences), which was modified by inserting an optimized red fluorescent protein variant (DsRed.T1) coding sequence followed by a rabbit beta-globin polyA signal into the first exon of the NG2 gene. The NG2 translation initiation ATG was changed to AAG to prevent translation from the BAC NG2 gene and to allow translation from the first ATG in the DsRed.T1 sequence.
  • mutations:
  • Insertion
  • synonyms:
  • NG2-DsRed,
  • NG2DsRedBAC,
  • NG2 dsRED,
  • NG2-DsRed,
  • NG2DsRedBAC,
  • NG2 dsRED
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1 Feature

Genome

0 Expresses

0 Mutation Involves

Phenotype

Mouse alleles --> Mammalian phenotypes (MP terms)

 

Other

2 Carried By

0 Driven By

117 Publication categories