| Primary Identifier | MGI:3800038 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Efhd2 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-Regeneron |
| description | Cell line 10332A-D1 was successfully used to make chimeric mice. Germline transmission was accomplished. J:228565 |
| molecularNote | The insertion of Velocigene cassette ZEN-Ub1 created a deletion of size 15139bp between positions 141415641-141430779 of Chromosome 4 (Genome Build37). The lacZ reporter gene and neomycin resistance selection cassette replaced the coding sequence. Western blot analysis confirmed the absence of protein expression in bone marrow, spleen and thymus. |
