| Primary Identifier | MGI:3802947 | Allele Type | Targeted |
| Attribute String | Inserted expressed sequence | Gene | Hbb |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | In ES cells that were homozygous for Hbatm1(HBA)Tow and Hbbtm2(HBG1,HBB*)Tow the human gamma chain and beta chain containing the sickle cell mutation were replaced with 4.6 kb fragment of human HBB containing nucleotide substitutions that lead to an amino acid substitution of alanine for cysteine at position 93 of human HBB. Flanking sequence that extends to -815 of HBB as well as a floxed hygro cassette were also inserted. Cre-mediated recombination was used to remove the hygro cassette. |
