| First Author | Servert P | Year | 2008 |
| Journal | Biotechniques | Volume | 45 |
| Issue | 1 | Pages | 69-78 |
| PubMed ID | 18611169 | Mgi Jnum | J:137580 |
| Mgi Id | MGI:3801253 | Doi | 10.2144/000112826 |
| Citation | Servert P, et al. (2008) In vivo site-specific recombination using the beta-rec/six system. Biotechniques 45(1):69-78 |
| abstractText | The prokaryotic beta serine recombinase (beta-rec) catalyzes site-specific recombination between two directly oriented six sites (93 bp) in mammalian cells, both in episomal and in chromosomally integrated substrates. The beta-rec/six exclusive intramolecular site-specific recombination (SSR) system has been proposed as a suitable approach when several independently controlled recombination events are needed in a single cell. Here we explored the use of the beta-rec/six system for selective induction of genome-targeted modifications. We generated and analyzed mouse transgenic lines (Tgbeta) expressing beta-rec under the control of the Lck promoter. beta-rec activity was demonstrated, and there was no evidence of alterations to thymic or peripheral T cell development. We developed two transgenic mouse lines harboring different target sequences (Tgrec and KOsix) and analyzed the effect of beta-rec expression on these animals. The results indicate that the beta-rec/six SSR system is functional for in vivo gene-targeting applications. |
