| First Author | Daum P | Year | 2022 |
| Journal | Front Immunol | Volume | 13 |
| Pages | 991347 | PubMed ID | 36591274 |
| Mgi Jnum | J:332443 | Mgi Id | MGI:7424084 |
| Doi | 10.3389/fimmu.2022.991347 | Citation | Daum P, et al. (2022) The microRNA processing subunit DGCR8 is required for a T cell-dependent germinal center response. Front Immunol 13:991347 |
| abstractText | We have previously shown that the microRNA (miRNA) processor complex consisting of the RNAse Drosha and the DiGeorge Critical Region (DGCR) 8 protein is essential for B cell maturation. To determine whether miRNA processing is required to initiate T cell-mediated antibody responses, we deleted DGCR8 in maturing B2 cells by crossing a mouse with loxP-flanked DGCR8 alleles with a CD23-Cre mouse. As expected, non-immunized mice showed reduced numbers of mature B2 cells and IgG-secreting cells and diminished serum IgG titers. In accordance, germinal centers and antigen-specific IgG-secreting cells were absent in mice immunized with T-dependent antigens. Therefore, DGCR8 is required to mount an efficient T-dependent antibody response. However, DGCR8 deletion in B1 cells was incomplete, resulting in unaltered B1 cell numbers and normal IgM and IgA titers in DGCR8-knock-out mice. Therefore, this mouse model could be used to analyze B1 responses in the absence of functional B2 cells. |
