Primary Identifier | MGI:3805020 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Wnt9b |
Transmission | Germline | Strain of Origin | 129X1/SvJ |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to insert a loxP site just upstream of exon 2, and a frt-flanked PGK-Neo cassette followed by a second loxP site just downstream of exon 2 of the targeted gene to generate Wnt9bMtm1Amc mice (Wnt9bcneo. To remove the frt-flanked selection cassette, these mice were then bred to an FLPe-expressing strain (on the Swiss-Webster genetic background). The resulting Wnt9bc offspring carry a loxP site just upstream of exon 2, and a single remaining frt-site followed by a second loxP site just downstream of exon 2. |