| Primary Identifier | MGI:3806292 | Allele Type | Transgenic |
| Attribute String | Inducible, Inserted expressed sequence, Reporter | Gene | Tg(tetO-SOD1*G93A,-luc)1Roos |
| Strain of Origin | Not Specified | Induced With | doxycycline/tetracycline |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A luciferase coding sequence and cDNA encoding human SOD1 with a G93A amino acid substitution were cloned into a plasmid containing a bi-cistronic tetracycline operator promoter (BiTetO). The construct was injected into micronuclei. Five lines were generated. Transgene expression was determined by luciferase activity in isolated fibroblasts transfected with a CMV-tTA plasmid. Analysis using anti-SOD1 and -luciferase antibodies was also performed on fibroblasts. |
