| Primary Identifier | MGI:3808120 | Allele Type | Targeted |
| Gene | Tal1 | Transmission | Germline |
| Strain of Origin | 129P2/OlaHsd | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Three highly conserved DNA-binding residues encoded in exon 6 (Arg-Glu-Arg at positions 195-197) were mutated to alanine residues. A floxed neo cassette was also inserted upstream of exon 6. Transient expression of Cre recombinase excised the neo cassette leaving behind a loxP site. The mutant protein was expressed at endogenous levels in the fetal liver, was localized to cellular nuclei, but lacked the ability to bind DNA. |
