| First Author | Koizumi M | Year | 2022 |
| Journal | FASEB J | Volume | 36 |
| Issue | 12 | Pages | e22662 |
| PubMed ID | 36412518 | Mgi Jnum | J:333188 |
| Mgi Id | MGI:7434839 | Doi | 10.1096/fj.202201002RR |
| Citation | Koizumi M, et al. (2022) UTX deficiency in neural stem/progenitor cells results in impaired neural development, fetal ventriculomegaly, and postnatal death. FASEB J 36(12):e22662 |
| abstractText | Recent studies have demonstrated that epigenetic modifications are deeply involved in neurogenesis; however, the precise mechanisms remain largely unknown. To determine the role of UTX (also known as KDM6A), a demethylase of histone H3K27, in neural development, we generated Utx-deficient mice in neural stem/progenitor cells (NSPCs). Since Utx is an X chromosome-specific gene, the genotypes are sex-dependent; female mice lose both Utx alleles (Utx(Delta/Delta) ), and male mice lose one Utx allele yet retain one Uty allele, the counterpart of Utx on the Y chromosome (Utx(Delta/Uty) ). We found that Utx(Delta/Delta) mice exhibited fetal ventriculomegaly and died soon after birth. Immunofluorescence staining and EdU labeling revealed a significant increase in NSPCs and a significant decrease in intermediate-progenitor and differentiated neural cells. Molecular analyses revealed the downregulation of pathways related to DNA replication and increased H3K27me3 levels around the transcription start sites in Utx(Delta/Delta) NSPCs. These results indicate that UTX globally regulates the expression of genes required for proper neural development in NSPCs, and UTX deficiency leads to impaired cell cycle exit, reduced differentiation, and neonatal death. Interestingly, although Utx(Delta/Uty) mice survived the postnatal period, most died of hydrocephalus, a clinical feature of Kabuki syndrome, a congenital anomaly involving UTX mutations. Our findings provide novel insights into the role of histone modifiers in neural development and suggest that Utx(Delta/Uty) mice are a potential disease model for Kabuki syndrome. |
