| First Author | Forte E | Year | 2020 |
| Journal | STAR Protoc | Volume | 1 |
| Issue | 2 | Pages | 100077 |
| PubMed ID | 33000003 | Mgi Jnum | J:354989 |
| Mgi Id | MGI:7734849 | Doi | 10.1016/j.xpro.2020.100077 |
| Citation | Forte E, et al. (2020) Protocol for Isolation of Cardiac Interstitial Cells from Adult Murine Hearts for Unbiased Single Cell Profiling. STAR Protoc 1(2):100077 |
| abstractText | Interstitial cells have a crucial role in cardiac fibrosis and repair of the mammalian heart. Single-cell profiling using droplet-based technology has revolutionized the investigation of cell states and identities. Here, we present a protocol for the efficient isolation of high-quality live nucleated non-cardiomyocytes from adult murine heart, for unbiased single-cell RNA sequencing using 10x Chromium technology. This protocol has been applied to homeostatic and injured hearts from different mouse strains. For complete details on the use and execution of this protocol, please refer to Forte et al. (2020). |
