| First Author | Novais EJ | Year | 2019 |
| Journal | Matrix Biol | Volume | 82 |
| Pages | 54-70 | PubMed ID | 30811968 |
| Mgi Jnum | J:282283 | Mgi Id | MGI:6378395 |
| Doi | 10.1016/j.matbio.2019.02.004 | Citation | Novais EJ, et al. (2019) p16(Ink4a) deletion in cells of the intervertebral disc affects their matrix homeostasis and senescence associated secretory phenotype without altering onset of senescence. Matrix Biol 82:54-70 |
| abstractText | Intervertebral disc degeneration is an important contributor to chronic low back and neck pain. Although many environmental and genetic factors are known to contribute to disc degeneration, age is still the most significant risk factor. Recent studies have shown that senescence may play a role in age-related disc degeneration and matrix catabolism in humans and mouse models. Clearance of p16(Ink4a)-positive senescent cells reduces the degenerative phenotype in many age-associated diseases. Whether p16(Ink4a) plays a functional role in intervertebral disc degeneration and senescence is unknown. We first characterized the senescence status of discs in young and old mice. Quantitative histology, gene expression and a novel p16(tdTom) reporter mice showed an increase in p16(Ink4a), p21 and IL-6, with a decrease in Ki67 with aging. Accordingly, we studied the spinal-phenotype of 18-month-old mice with conditional deletion of p16(Ink4a) in the disc driven by Acan-CreERT2 (cKO). The analyses of discs of cKO and age-matched control mice showed little change in cell morphology and tissue architecture. The cKO mice exhibited changes in functional attributes of aggrecan as well as in collagen composition of the intervertebral disc. While cKO discs exhibited a small decrease in TUNEL positive cells, lineage tracing experiments using ZsGreen reporter indicated that the overall changes in cell fate or numbers were minimal. The cKO mice maintained expression of NP-cell phenotypic markers CA3, Krt19 and GLUT-1. Moreover, in cKO discs, levels of p19(Arf) and RB were higher without alterations in Ki67, gammaH2AX, CDK4 and Lipofuscin deposition. Interestingly, the cKO discs showed lower levels of SASP markers, IL-1beta, IL-6, MCP1 and TGF-beta1. These results show that while, p16(Ink4a) is dispensable for induction and maintenance of senescence, conditional loss of p16(Ink4a) reduces apoptosis, limits the SASP phenotype and alters matrix homeostasis of disc cells. |
