| First Author | Pan Y | Year | 2023 |
| Journal | Neurosci Bull | Volume | 39 |
| Issue | 12 | Pages | 1762-1774 |
| PubMed ID | 37233921 | Mgi Jnum | J:351381 |
| Mgi Id | MGI:7702382 | Doi | 10.1007/s12264-023-01069-y |
| Citation | Pan Y, et al. (2023) Fgf8(P2A-3xGFP/+): A New Genetic Mouse Model for Specifically Labeling and Sorting Cochlear Inner Hair Cells. Neurosci Bull 39(12):1762-1774 |
| abstractText | The cochlear auditory epithelium contains two types of sound receptors, inner hair cells (IHCs) and outer hair cells (OHCs). Mouse models for labelling juvenile and adult IHCs or OHCs exist; however, labelling for embryonic and perinatal IHCs or OHCs are lacking. Here, we generated a new knock-in Fgf8(P2A-3xGFP/+) (Fgf8(GFP/+)) strain, in which the expression of a series of three GFP fragments is controlled by endogenous Fgf8 cis-regulatory elements. After confirming that GFP expression accurately reflects the expression of Fgf8, we successfully obtained both embryonic and neonatal IHCs with high purity, highlighting the power of Fgf8(GFP/+). Furthermore, our fate-mapping analysis revealed, unexpectedly, that IHCs are also derived from inner ear progenitors expressing Insm1, which is currently regarded as an OHC marker. Thus, besides serving as a highly favorable tool for sorting early IHCs, Fgf8(GFP/+) will facilitate the isolation of pure early OHCs by excluding IHCs from the entire hair cell pool. |
