| First Author | Holderith N | Year | 2021 |
| Journal | Front Synaptic Neurosci | Volume | 13 |
| Pages | 773209 | PubMed ID | 35221979 |
| Mgi Jnum | J:333739 | Mgi Id | MGI:7440131 |
| Doi | 10.3389/fnsyn.2021.773209 | Citation | Holderith N, et al. (2021) Selective Enrichment of Munc13-2 in Presynaptic Active Zones of Hippocampal Pyramidal Cells That Innervate mGluR1alpha Expressing Interneurons. Front Synaptic Neurosci 13:773209 |
| abstractText | Selective distribution of proteins in presynaptic active zones (AZs) is a prerequisite for generating postsynaptic target cell type-specific differences in presynaptic vesicle release probability (P(v)) and short-term plasticity, a characteristic feature of cortical pyramidal cells (PCs). In the hippocampus of rodents, somatostatin and mGluR1alpha expressing interneurons (mGluR1alpha+ INs) receive small, facilitating excitatory postsynaptic currents (EPSCs) from PCs and express Elfn1 that trans-synaptically recruits mGluR7 into the presynaptic AZ of PC axons. Here we show that Elfn1 also has a role in the selective recruitment of Munc13-2, a synaptic vesicle priming and docking protein, to PC AZs that innervate mGluR1alpha+ INs. In Elfn1 knock-out mice, unitary EPSCs (uEPSCs) in mGluR1alpha+ INs have threefold larger amplitudes with less pronounced short-term facilitation, which might be the consequence of the loss of either mGluR7 or Munc13-2 or both. Conditional genetic deletion of Munc13-2 from CA1 PCs results in the loss of Munc13-2, but not mGluR7 from the AZs, and has no effect on the amplitude of uEPSCs and leaves the characteristic short-term facilitation intact at PC to mGluR1alpha+ IN connection. Our results demonstrate that Munc13-1 alone is capable of imposing low P(v) at PC to mGluR1alpha+ IN synapses and Munc13-2 has yet an unknown role in this synapse. |
